ELISA Development Kitは、ELISAプレートをお客様ご自身で構築するキットで、ELISAプレートの構築に必要な補足用抗体、検出用抗体、スタンダードなどがセットされています。ELISAプレートの構築には少しの手間がかかりますが、Standardサイズでプレート約10枚分（約1,000ウェル分）を構築でき、固相化済みプレートの購入と比較すると大幅なコストダウンが期待できます。また、使用する分を作製できるため無駄が少なくなります。
製品容量は、約10枚分（約1,000ウェル分）のELISAプレートを構築できるStandardサイズの他にも、プレート約2枚分（約200ウェル分）のMiniサイズを用意している製品もあります。
詳細情報 ＞＞ Peprotech社WEBサイト
メーカー情報
Enzyme-linked immunosorbent assay (ELISA) is a laboratory technique for quantifying the concentration of specific antigens or antibodies in cell lysates, sera, or other complex solutions. PeproTech’s ELISA Development Kits contain the key components required for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. Each kit contains the capture antibody, a biotinylated detection antibody, a calibrated antigen standard, the Avidin Peroxidase conjugate, and a kit-specific protocol.
■ ELISAプレート構築プロトコール
■ キット構成 例）Human IL-6 ELISA Development Kit
- Capture Antibody:
100 μg of antigen-affinity purified goat anti-hIL-6 + 2.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 ml sterile water for a concentration of 100 μg/ml. - Detection Antibody:
25 μg of biotinylated antigen-affinity purified goat anti-hIL-6 + 2.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 0.25 ml sterile water for a concentration of 100 μg/ml. - Human IL-6 Standard:
1 μg of recombinant hIL-6 + 2.2 mg BSA + 11.0 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 ml sterile water for a concentration of 1 μg/ml. - Avidin-HRP Conjugate:
60 μl vial. Upon receipt, avidin-HRP conjugate should be aliquoted into ten 6 μl vials and stored at <-200C. Aliquots stored frozen at <-200C are stable for up to 2 years form date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.
■ 製品ラインアップ
● Human ELISA Development Kit
● Murine ELISA Development Kit
| Standard 約1,000ウェル分 74,000円 |
Mini 約200ウェル分 23,000円 |
|
| EGF | 900-K179 | |
| Exodus-2 | 900-K132 | 900-M132 |
| G-CSF | 900-K103 | |
| GM-CSF | 900-K55 | 900-M55 |
| IFN-γ | 900-K98 | 900-M98 |
| IGF-I | 900-K170 | |
| IL-1α | 900-K82 | |
| IL-1β | 900-K47 | 900-M47 |
| IL-2 | 900-K108 | 900-M108 |
| IL-3 | 900-K48 | 900-M48 |
| IL-4 | 900-K49 | 900-M49 |
| IL-5 | 900-K406 | 900-M406 |
| IL-6 | 900-K50 | 900-M50 |
| IL-10 | 900-K53 | 900-M53 |
| IL-12 | 900-K97 | 900-M97 |
| IL-13 | 900-K207 | 900-M207 |
| IL-15 | 900-K188 | 900-M188 |
| IL-17 | 900-K392 | 900-M392 |
| IL-21 | 900-K368 | 900-M368 |
| IL-22 | 900-K257 | 900-M257 |
| IP-10 | 900-K153 | 900-M153 |
| JE/MCP-1 | 900-K126 | 900-M126 |
| KC | 900-K127 | 900-M127 |
| Leptin | 900-K76 | |
| MCP-3 | 900-K123 | |
| M-CSF | 900-K245 | |
| MDC | 900-K197 | |
| MIP-1α | 900-K125 | 900-M125 |
| MIP-1β | 900-K278 | 900-M278 |
| MIP-2 | 900-K152 | 900-M152 |
| sRANK Ligand | 900-K233 | 900-M233 |
| RANTES | 900-K124 | 900-M124 |
| SCF | 900-K78 | |
| TNF-α | 900-K54 | 900-M54 |
| VEGF | 900-K99 | 900-M99 |
● Rat ELISA Development Kit
| Standard 約1,000ウェル分 74,000円 |
Mini 約200ウェル分 23,000円 |
|
| CNTF | 900-K65 | 900-M65 |
| EGF | 900-K390 | 900-M390 |
| GRO/KC | 900-K57 | |
| IFN-γ | 900-K109 | |
| IL-1α | 900-K204 | |
| IL-1β | 900-K91 | 900-M91 |
| IL-2 | 900-K205 | 900-M205 |
| IL-6 | 900-K86 | 900-M86 |
| IP-10 | 900-K449 | 900-M449 |
| MCP-1 | 900-K59 | 900-M59 |
| MIP-1α | 900-K75 | |
| RANTES | 900-K72 | 900-M72 |
| SCF | 900-K258 | 900-M258 |
| TNF-α | 900-K73 | |
| VEGF | 900-K436 | 900-M436 |
● ELISA Buffer Kit
Catalog Number: 900-K00
- PBS:
2 x 10ml per bottle 20x concentrated PBS solution - Wash Buffer:
2 x 250ml per bottle 20x concentrated Wash Buffer (1.0% TWEEN-20 in PBS) - Blocking Buffer:
2 x 200ml per bottle ready to use Blocking Buffer (1% BSA in PBS) - Diluent:
2 x 15ml per bottle 20x concentrated Diluent (1.0% TWEEN-20, 2.0% BSA in PBS) - ABTS Liquid Substrate:
2 x 60ml per bottle ready to use ABTS - ELISA Microplates:
10 x 96 well plates (Corning Product #3590) - Sealing Film:
50 Polyester Adhesive Sheets
詳細情報 ＞＞ Peprotech社WEBサイト
