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Media of Choice for Adherent & Organotypic Neural Stem Cell Culture
RHB-A is a proprietary, defined, serum-free cell culture medium that has been specifically formulated for the culture of pure populations of adherent human and mouse NS cells. This optimized medium ensures the stable maintenance of NS cell differentiation capacity after prolonged culture in vitro1.
RHB-A has also been demonstrated to:
- Support the differentiation of human and mouse NS cells into functional neurons, with recorded neuronal differentiation of greater than 40%1,2,9
- Improve the efficiency of neural differentiation of mouse embryonic stem (ES) cells in monolayer culture with recorded differentiation to greater than 80% neural precursors4,7,8
- Derive, maintain and expand human glioblastoma stem cell lines6
- Culture primary human brain tissue in vitro with radial glia derived neurogenesis9
Applications
Neural Cell Culture
For the derivation, maintenance and expansion of adherent human and mouse NS cells1,3,5, RHB-A should be supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2). Key performance attributes of RHB-A include:
- Maintenance of adherent NS cells at ≥ 99% purity
- Continual propagation of NS cells without loss of neurogenic capacity1
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Neural Differentiation
NS to neurons
Sequential withdrawal of the EGF and the FGF-2 growth factor supplements from RHB-A can enable the efficient differentiation of greater than 40% of adherent human NS and mouse NS cells into neurons1, 2.
Differentiated human NS cells
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ES to neurons
The use of RHB-A without growth factor supplements has been shown to enable improved differentiation of mouse ES cells when plated in adherent monolayer conditions4,7. Efficiencies of greater than 80% differentiation of mouse ES cells into neural precursors have been recorded.
Monolayer differentiation of ES cells to neurons
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ES cells ES-derived neurons
![feature-rhb_esCells.jpg]()
Glioblastoma Stem Cell Culture
RHB-A has been demonstrated to support the maintenance and expansion of glioblastoma stem cells when supplemented with EGF and FGF-26.
RHB-Basal
To provide maximum flexibility for your research needs, we also provide a basal formulation of the RHB-A medium called RHB-Basal. This proprietary basal formulation does not contain any neuronal supplements, thereby enabling you to tailor RHB-Basal to suit the specific requirements of your cell type through the addition of your choice of supplements.
| Product Name |
Cat. No. |
Amount |
| RHB-A |
SCS-SF-NB-01 |
500 ml |
| RHB-Basal |
SCS-SF-NB-00 |
500 ml |
References
1. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Molecular and Cellular Neuroscience 38:245-258
2. Conti L, et al. (2005) Niche- Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology 3(9):e283
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120
4. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nature Biotechnology 21:183-186
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403
6. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 4:568-580
7. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnology and Applied Biochemistry 49:105-112
8. Abranches E, et al. (2009) Neural differentiation of embronic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE 4(7): e6286
9. Hansen DV, et al. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464(7288): 554-561
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