初代神経細胞用培地 NbActiv4®
NbActiv4®は、Neurobasal®/B27®を改良した神経細胞用培地です。Neurobasal®/B27®にクレアチン、エストロゲン、コレステロールの3つの組成を添加することにより、スパイク発火率が最大限になるように最適化されています。Neurobasal®/B27®よりも2倍のシナプスと8倍までの高いスパイク発火率を持つ神経細胞に成長します。
■ メーカー情報
NbActiv4® neuron culture medium is an improved version of Neurobasal®/B27®. NbActiv4® grows neurons with twice as many synapses and up to 8x higher spike rates than Neurobasal®/B27®.
NbActiv4® was optimized for maximum spike rates by addition of 3 new components to
Neurobasal®/B27®: creatine, estrogen and cholesterol. Measures of respiration indicated that neurons in NbActiv4® were under less metabolic stress and operated more efficiently under demand loading from glutamate. These results suggest that NbActiv4® will be a convenient and useful serum-free neuron culture medium for electrophysiology, developmental synaptic plasticity, neuronal network analyses, pharmacology, toxicology and drug discovery. Synapses and spiking activity develop earlier, starting at 1 week in NbActiv4® compared to Neurobasal®/B27® (Brewer et al. 2008. Neuron network activity scales exponentially with synapse density. J. Neural Eng., in press) Brewer,GJ., Boehler, MD; Jones, TT, Wheeler, BC. 2008. NbActiv4 medium for neurons increases synaptic densities and network spike rates on multielectrode arrays compared to Neurobasal/B27. J. Neurosci. meth. 170:181-187 (PM 18308400)
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