遺伝子（cDNA/ORF）発現レンチウイルスベクター作製用プラスミド
ご好評いただいていますTrueORF（cDNA/ORF発現ベクター）にレンチウイルスベクター作製用プラスミドが新しくラインアップされました。
cDNA/ORF配列がレンチウイルスベクター作製用プラスミドへサブクローニングされており、プラスミドをパッケージング細胞へトランスフェクションすることで遺伝子発現レンチウイルスベクターを作製することができます。C末端にc-Myc-DDKタグを融合して発現させるpLenti-C-Myc-DDK、緑色蛍光タンパク質mGFPを融合して発現させるpLenti-C-mGFPの2タイプから選択いただけます。
通常のTrueORF検索で、製品ラインアップやベクター配列・マップなどの詳細情報が確認できます。
http://www.origene.com/orf/
■ メーカー情報
pLenti-C-Myc-DDK
This Lenti vector is a third generation of lentiviral vector.
- All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
- The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags
- Lentiviral infection is a convenient way to delivery DNA into most cell lines and is uniquely effective for the non-dividing cells.
- A brief lentiviral packaging protocol is provided.
Schematic of the multiple cloning sites:
pLenti-C-mGFP
This Lenti vector is a third generation of lentiviral vector.
- All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
- The ORF cloned in this vector can be expressed as a tagged protein with a c-terminal monomeric GFP tag.
- mGFP (scientific name mTagGFP) is the improved variant of tagGFP, the mutant of the Aequorea macrodactyla GFP-like protein. mGFP possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively. Compared to EGFP, mGFP provides about the same brightness of fluorescence but is significantly more pH stable. mGFP is specially optimized for expression at 37°C. Because of monomeric nature, mGFP is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, mGFP can be used as a donor for red fluorescent protein mRFP or as an acceptor for blue fluorescent protein mBFP.
- A brief lentiviral packaging protocol is provided.
Schematic of the multiple cloning sites:
