RNAi研究に！ shRNA発現レンチウイルスベクター作製用プラスミド
ご好評いただいていますRNAi研究用のHuSH-29（shRNA発現ベクター）にレンチウイルスベクター作製用プラスミドが新しくラインアップされました。
shRNA配列がレンチウイルスベクター作製用プラスミドへサブクローニングされており、プラスミドをパッケージング細胞へトランスフェクションすることでshRNA発現レンチウイルスベクターを作製することができます。shRNA配列は緑色蛍光タンパク質tGFPでモニタリングおよびPuromycinでセレクション可能なpGFP-C-shLentiに組み込まれています。その他、カスタムサービスで、赤色蛍光タンパク質tRFPでモニタリングおよびBlasticidinでセレクション可能なpRFP-CB-shLentiに組み込むこともできます。
通常のHuSH-29検索で、製品ラインアップやベクター配列・マップなどの詳細情報が確認できます。
http://www.origene.com/shRNA/
■ メーカー情報
pGFP-C-shLenti
pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
For our RNAi products, the shRNA expression cassette consists of a 29 bp target gene specific sequence, a 7 bp loop, and another 29 bp reverse complementary sequence, all under human U6 promoter. A termination sequence (TTTTTT) is located immediately downstream of the second 29 bp reverse complementary sequence to terminate the transcription by RNA Pol III. The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.
pRFP-CB-shLenti
pRFP-CB-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a blasticidin resistant gene driven by a SV40 promoter and a tRFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
