リン酸化プロテオーム解析のためのリン酸化ペプチドの濃縮におすすめ
Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful mass spectrometry - based phosphoproteomics experiments. PolyMAC o ffers an efficient and greatly improved method to achieve more complete phosphopeptide enrichment under homogeneous conditions. This highly selective enrichment procedure can be used with majority of the complex samples because it provides optimal specific ity toward phosphopeptides.
After in-solution binding of phosphopeptides, PolyMAC needs to be captured covalently onto solid-phase beads to complete washing and elution steps. We offer capture based on magnetic beads (Recommended) or agarose beads and spin columns.
All kits include PolyMAC-Ti reagent, capture beads, loading buffer, washing buffer, capture buffer and elution buffer. Agarose capture-based kits also include re-usable spin columns.
Performance comparison.
100ug of digested complex cell lysate s from DG - 75 B - cell lymphoma cells with different phosphorylation levels were used to compare the enrichmen t efficiency between titanium - based PolyMAC and other commercially available reagents. A number of unique phosphosites identified and percent enrichment selectivity were used as the parameters for comparison .
Original citation.
Iliuk AB, Martin VA, Alicie BM, Geahlen RL and Tao WA (2010). In - depth analyses of kinase - dependent tyrosine phosphoproteomes based on metal ion functionalized soluble nanopolymers. Mol Cell Proteomics. 9 : 2162 - 72.