リン酸化タンパク質をウェスタンブロッティング（WB）で感度よく検出
pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho - antibodies, the binding is not biased by amino acid sequence, and therefore can be used for detection of any phosphorylation event on any protein site. pIMAGO detection protocol resembles a simple Western Blot procedure and can be easily incorporated by any laboratory.
Through extensive testing, pIMAGO demonstrates the following advantages:
-Sensitive & specific universal detection of phosphoproteins
-Good quantitative capabilities and linearity
-Effectiveness for in vitro kinase and phosphatase assays
-Ability to detect in vivo phosphorylated proteins after immunoprecipitation
-Efficacy for kinase activity profiling and inhibitor screening
-Multiplexed detection capabilities of pIMAGO with total protein antibodies
Results.
pIMAGO analysis of 6 protein mixture (2 phosphoproteins) avidin - HRP or avidin - Fluor detection
Original citation.
Iliuk A., Liu XS., Xue L., Liu X., Tao WA. (2012). Chemical visualization of phosphoproteomes on membrane. Molecular and Cellular Proteomics. 11: 629-639. doi: 10.1074/mcp.O112.018010