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GLYOXAL RESINS
Immobilization technique allows affinity ligands’ binding giving high stability and making easy to re-use the conjugated ligand. Glyoxal beads allow a covalent binding of agarose to lysine amino groups.
ABT offers a wide range of products activated with different degree of aldehydes groups and using two different backbone (4% & 6% beads) and different particle sizes. Giving adequate options to work in batch or column purifications (Glyoxal Agarose Beads for Low Pressure applications) and large scale process (Glyoxal Rapid Run™ for High Pressure applications).
ABT Glyoxal resins allow a covalent binding of agarose to amino groups of ligands (antibodies, antigens, enzymes, proteins or other biomolecules). The aldehyde groups (resin) react with exposed primary amines (biomolecul). The result of the biomolecule immobilization is a stable and reusable resin for affinity purification in batch, spin column or gravity procedures.
COUPLING LIGAND
Ligand: antibody, antigen, enzyme, affinity proteins (e.g. Protein A or Protein G) or biomolecule.
FEATURE
- Binding through Lys zones: reproducible process
- Very stable ligand binding (covalent )
- High ligand binding capacity
- High specificity (no cations present)
- Quick ligand conjugation
- No affinity ligand loss (reusable)
- Ready to use
- Long shelf- life (expiration 5 years)
Low Pressure: GLYOXAL Agarose Beads
| Bead (Geometry, size) | Spherical, Standard: ～50 - 150µm | |||||
| Cross-Linked | Yes | |||||
| Matrix Active Groups | Agarose with some diols oxidized to aldehydes | |||||
| Product Name | VERY HIGH Density GLYOXAL 4BCL | VERY HIGH Density GLYOXAL 6BCL | HIGH Density GLYOXAL 4BCL | HIGH Density GLYOXAL 6BCL | LOW Density GLYOXAL 4BCL | LOW Density GLYOXAL 6BCL |
| Agarose % | 4 | 6 | 4 | 6 | 4 | 6 |
| Activation Degree (µmol Glyoxyl/ml gel) | - | 80-100 | 40-60 | 40-60 | 15-25 | 15-25 |
| Binding Capacity(1) (mg BSA/ml gel) | - | 25-30 | 15-20 | 20-25 | 5-10 | 5-10 |
| Cat. No. | - | 6BCL-GH1-X* | 4BCL-GH1-X* | 6BCL-GM3-X* | 4BCL-GH0-X* | 6BCL-GH0-X* |
| Antimicrobial Agent | 0.5M NaCL containing 0.02% thimerosal or 20% ethanol | |||||
| Storage Temperature | 2-8℃ | |||||
*X: Product Quantity
High Pressure: GLYOXAL Rapid Run™ Beads
| Bead (Geometry, size) | Spherical, Standard: ～50 - 150µm | Spherical, Fine: ～20 - 50µm | |||||
| Cross-Linked | Highly cross-linked agarose | ||||||
| Matrix Active Groups | Agarose with some diols oxidized to aldehydes | ||||||
| Product Name | High Density GLYOXAL 4 RAPID RUNTM | High Density GLYOXAL 6 RAPID RUNTM | LOW Density GLYOXAL 4 RAPID RUNTM | LOW Density GLYOXAL 6 RAPID RUNTM | High Density GLYOXAL 4 RAPID RUNTM FINE | High Density GLYOXAL 4 RAPID RUNTM 5 atoms | High Density GLYOXAL 6 RAPID RUNTM FINE |
| Agarose % | 4 | 6 | 4 | 6 | 4 | 4 | 6 |
| Activation Degree (µmol Glyoxyl/ml gel) | 40-60 | 40-60 | 15-25 | 15-25 | 15-25 | ～15 | 15-25 |
| Cat. No. | 4RR-GH1-X* | 6RR-GM3-X* | 4RR-GL0-X* | 6RR-GL0-X* | 4RRF-GL0-X* | 4BRRF-5L-X* | 6RRF-GL0-X* |
| Antimicrobial Agent | 20% Ethanol | ||||||
| Storage Temperature | 2-8℃ | ||||||
*X: Product Quantity (25 or 100ml)