Polysciences社では、酵素固定化用のレジンを販売しています。レジンにはアクリル樹脂とスチレン樹脂を採用し、酵素を結合・吸着させる官能基にはエポキシ基、アミノ基、オクタデシル基をそれぞれラインアップしています。担体に固定化された酵素は、操作面では酵素反応後の分離が容易であり、経済面では酵素を繰り返し利用できるなどの利点があります。
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Immobilized enzymes are powerful tools to optimize processes in both operative and economic terms.
In addition to a more convenient handling of enzyme preparations, the two main targeted benefits of immobilized enzymes are 1) easy separation of the enzyme from the product, and 2) reuse of the enzyme. Immobilized enzymes allow the easy separation of the catalyst after the reaction e.g. filtration thereby reducing the costs of downstream processing. Moreover, the easy separation of the enzyme from the product simplifies enzyme applications and enables a reliable and efficient reaction technology. On the other hand, the reuse of immobilized enzymes provides cost advantages which are often an essential prerequisite for establishing an enzyme-catalyzed process in the first place. Immobilized enzymes produce important pharmaceuticals and food additives in large ton scale.
■ Epoxy Functionalized
Epoxy-activated resins are almost ideal matrices to perform easy immobilization of enzymes since they allow multipoint covalent binding between the enzyme and resin.
- ECR4204F, Epoxy Acrylate/Styrene Enzyme Carrier Resin
- ECR4204M, Epoxy Acrylate/Styrene Enzyme Carrier Resin
- ECR8205F, Epoxy Acrylate Enzyme Carrier Resin
- ECR8205M, Epoxy Acrylate Enzyme Carrier Resin
- ECR8214F, Epoxy Acrylate Enzyme Carrier Resin
- ECR8214M, Epoxy Acrylate Enzyme Carrier Resin
- PolyLink Epoxy Linker Kit - F50
- PolyLink Epoxy Linker Kit with Columns and Reagents for Enzyme Immobilization
■ Amine Functionalized
Another procedure for covalent immobilization of enzymes is based on the use of amino resins. Amino resins can be pre-activated by glutaraldehyde and then used in the covalent immobilization of enzymes (see Figure 4). Reaction of the aldehyde groups with amino groups of enzymes to form Schiff bases is fast and gives stable multipoint covalent binding. An even more stable linkage can be achieved by reduction with borohydrides.
- ECR8310F, Amino C2 Acrylate Enzyme Carrier Resin
- ECR8310M, Amino C2 Acrylate Enzyme Carrier Resin
- ECR8319F, Amino C2 Acrylate Enzyme Carrier Resin
- ECR8319M, Amino C2 Acrylate Enzyme Carrier Resin
- ECR8405F, Amino C6 Acrylate Enzyme Carrier Resin
- ECR8405M, Amino C6 Acrylate Enzyme Carrier Resin
- ECR8417F, Amino C6 Acrylate Enzyme Carrier Resin
- ECR8417M, Amino C6 Acrylate Enzyme Carrier Resin
- PolyLink Amine Linker Kit - F50
■ Resins for Enzyme Adsorption
This method for the immobilization of enzymes is based on the physical adsorption of enzyme protein on the surface of water-insoluble carriers. The method is very gentle and causes little or no conformational change of the enzyme or destruction of its active center. This method is particularly suitable for applications in organic solvents or hydrophobic media such oils. A major advantage of adsorption as a general method of immobilizing enzymes is that usually no reagents are required.
- ECR1090F, Macroporous Styrene Enzyme Carrier Resin
- ECR1090M, Macroporous Styrene Enzyme Carrier Resin
- ECR1091F, Macroporous Styrene Enzyme Carrier Resin
- ECR1091M, Macroporous Styrene Enzyme Carrier Resin
- ECR8804F, Octadecyl Acrylate Enzyme Carrier Resin
- ECR8804M, Octadecyl Acrylate Enzyme Carrier Resin
- ECR8806F, Octadecyl Acrylate Enzyme Carrier Resin
- ECR8806M, Octadecyl Acrylate Enzyme Carrier Resin
- PolyLink Enzyme Adsorption Kit - F50