Lipid Snoopers®は高純度の脂質がニトロセルロースメンブレン上にスポットされています。製品ラインアップは、ホスファチジルイノシトールでまとめられたInositol Snoopers®、酸化リン脂質でまとめられたOxidized Phospholipid Snoopers®の2種類があります。細胞シグナル伝達関連分子の同定、脂質結合抗体の特性分析、新規脂質結合タンパク質の同定などに利用できます。
詳細情報 ＞＞ Avanti Polar Lipids社WEBサイト
メーカー情報
■ Inositol Snoopers®
Avanti Inositol Snoopers consist of 13 different lipid species spotted individually on a solid support. Each spot contains 1 µg of the highest quality pure lipid and is ideal for investigating lipid-protein interactions.
Avanti Snoopers have been used as a method to determine/confirm the specificity of Avanti’s WR304 antibody, which recognizes PIP and PIP2.
Inositol Snoopers were blocked with 3% BSA (fatty acid free) in TBS (0.8% NaCl, 20 mM Tris-HCL pH 7.4) containing 1 mM EDTA. The blocked membranes were then probed with WR304 (1.0 μg/mL) in TBS containing 1% BSA and 1 mM EDTA for 1 h at RT. After washing the membranes 3 times with TBS containing 1 mM EDTA the bound antibody was detected with goat antimouse IgM conjugated to horseradish peroxidase (HRP) (Southern Biotech) at a 1:10,000 dilution in TBS containing 1% BSA and 1 mM EDTA for 1 h at RT. After washing, bound HRP was visualized on an X-ray film with an enhanced chemiluminescence substrate (Pierce).
詳細情報 ＞＞ Avanti Polar Lipids社WEBサイト
■ Oxidized Phospholipid Snoopers®
Avanti Ox-Phospholipid Snoopers consist of 12 different lipid species spotted individually on a solid support. Each spot contains 1 µg of the highest quality pure lipid and is ideal for investigating lipid-protein interactions.
conjugated to horseradish peroxidase (HRP) (Southern Biotech) at a 1:10,000 dilution in TBS containing 1% BSA for 1 h at RT. After washing, bound HRP was visualized on an X-ray film with an enhanced chemiluminescence substrate (Pierce).
詳細情報 ＞＞ Avanti Polar Lipids社WEBサイト