適切なECM環境の選択に最適
The MicroMatrix™ cell culture system is an in vitro system for live cellular attachment of cells to 36 combinations of extracellular matrix protein composed microenvironments. Compositions have been miniaturized in the form of 400µm uniform circular spots to support a cell based assay. The system relies on a cell type of interests inherent affinity towards a particular ECM combination for adherence. Cell based assays may be performed directly on the system. Assays are end user defined and readouts may include standard fluorescence based imaging and whole cell colormetric staining. Assays can be real-time or end-point and can be perform
ed using standard cell culture equipment.
ECMP components of each block.
| Collagen I (250μg/ml) | Collagen I (125μg/ml) Collagen III (125μg/ml) |
Fibronectin (125μg/ml) Collagen IV (125μg/ml) |
| Collagen III (250μg/ml) | Collagen I (125μg/ml) Collagen IV (125μg/ml) |
Fibronectin (125μg/ml) Collagen VI (125μg/ml) |
| Collagen IV (250μg/ml) | Collagen I (125μg/ml) Collagen V (125μg/ml) |
Fibronectin (125μg/ml) Laminin (125μg/ml) |
| Collagen V (250μg/ml) | Collagen I (125μg/ml) Collagen VI (125μg/ml) |
Fibronectin (125μg/ml) Vitronectin(125μg/ml) |
| Collagen VI (250μg/ml) | Collagen I (125μg/ml) Fibronectin (125μg/ml) |
Laminin (125μg/ml) Collagen IV (125μg/ml) |
| Fibronectin (250μg/ml) | Collagen I (125μg/ml) Laminin (125μg/ml) |
Laminin (125μg/ml) Collagen VI (125μg/ml) |
| Laminin (250μg/ml) | Collagen I (125μg/ml) Vitronectin (125μg/ml) |
Vitronectin (125μg/ml) Collagen IV (125μg/ml) |
| Vitronectin (250μg/ml) | Collagen I (125μg/ml) Topoelastin (125μg/ml) |
Vitronectin (125μg/ml) Collagen VI (125μg/ml) |
| Tropoelastin (250μg/ml) | Fibronectin (83.3μg/ml) Laminin (83.3μg/ml) Collagen I (83.3μg/ml) |
Vitronectin (125μg/ml) Laminin (125μg/ml) |
| Collagen IV (125μg/ml) Collagen VI (125μg/ml) |
Fibronectin (83.3μg/ml) Laminin (83.3μg/ml) Collagen IV (83.3μg/ml) |
Vitronectin (125μg/ml) Topoelastin (125μg/ml) |
| Collagen III (125μg/ml) Collagen V (125μg/ml) |
Vitronectin (62.5μg/ml) Laminin (62.5μg/ml) Collagen I (62.5μg/ml) Collagen IV (62.5μg/ml) |
Topoelastin (125μg/ml) Collagen IV (125μg/ml) |
| Negative Control BSA (250μg/ml) |
Fibronectin (62.5μg/ml) Laminin (62.5μg/ml) Collagen I (62.5μg/ml) Collagen IV (62.5μg/ml) |
Topoelastin (125μg/ml) Collagen VI (125μg/ml) |
Concentrations indicated in parentheses represent the concentration of the ECMP in the solution that is spotted on the slide.Each block contains a unique ECMP condition with 9 replicate spots. Spot diameter is 400 μm. Distance between spots in each block is 500 μm from center to center.Each block is 4.5 mm in diameter.
Examples of cell attachment within a single condition (9 replicate spots) on a MicroMatrix™ 36 slide.
Left image is MCF 7 cells. Right image is human hepatocytes. Images were taken on a 5× objective lens on a Leica DMIL microscope system.
Reduced Protocol
In a sterile hood:
- Remove MicroMatrix slide and the four-chambered cell culture tray from package, do not touch the surface of the slide
- Place the slide in one of the chambers of the tray (logo on the slide should be bottom right)
- Add cells in 5ml media onto the slide
- Incubate cells (3 hours - 2 days) until sufficient cell attachement is observed
- Aspirate away unattached cells and perform user defined experiments
- Fix/stain/image/analyze (end user defined)
Kit Components
1. One 75mm x 25mm hydrogel coated glass slide pre-printed with 36 ECMP combinations of collagen I, collagen III, collagen IV, collagen V, collagen VI, fibronectin, laminin, vitronectin and tropoelastin in a slide case
2. One four-chambered slide culture tray
| Product Name | Catalog No | Details |
|
MicroMatrix™ 36 ECM Array |
MM012011 |
