高発現ベクターで、高純度のタンパク質精製が可能
Protein expression in mammalian cells has experienced an
increased usage within the last years. In contrast to other
expression systems (like E. coli) recombinant proteins
produced in mammalian expression systems can provide
more post-translational modifications, such as glycosylation,
which are a necessity for the functionality of the expressed
proteins.
IBA offers all essential components for efficient protein expression
in mammalian cells with the mammalian expression
system MEXi:
- HEK293E cell-line, cell-culture media and expression vector are perfectly matched to provide high protein yields without further optimization
- Transient expression for time and cost efficient protein production
- Efficient nuclear import and extrachromosomal replication due to vector encoded EBV origin of replication (oriP) and EBNA-1 provided by MEXi-293E cells
- Multiple expression vectors with different features available
- Twin-Strep-tag for highly pure proteins
The MEXi system combines high protein yields with high purity
Secreted alkaline phosphatase (SEAP) and a mAb, both fused with Twin-Streptag (TST), were expressed with the MEXi system and purified via the TST resulting in high yields of pure protein: 143 mg/l cell culture for SEAP and 96 mg/l for the mAb, heavy (HC) and light chain (LC).
pDSG-IBA expression vector:
Available with different tags, like Twin-Strep-tag, Strep-tag®II and 6xHis-tag and BM40 secretion signal.
| Cat. No. | Name |
| 5-52XX-000 | pDSG-IBA Expression vectors |
| 2-6010-010 | MEXi-CM Culture Medium |
| 2-6011-010 | MEXi-TM Transfection Medium |
| 2-6001-001 | MEXi-293E HEK293E cell line |
製品概要
http://www.iba-lifesciences.com/newsdetails/items/mexi-mammalian-expression-system-from-iba.html