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mCLING
A fixable membrane probe for high resolution microscopy
General Information
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.
Membrane staining of 3T3 fibroblasts with ATTO 647N-labeled mCLING.
| Cat. No. 710 006AT1 | 5nmol mCLING labeled with ATTO® 647N in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Cat. No. 710 006AT3 | 5nmol mCLING labeled with ATTO® 488 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Cat. No. 710 006DY1 | 5nmol mCLING labeled with DY® 654 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) 710 006AT1 710 006AT3 710 006DY1 IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml) |
References SySy mCLING
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T & Rizzoli SO (2014). A new probe for super-resolution imaging of membranes elucidates trafficking pathways. Journal of Cell Biology 205: 591-606.