Peprotech社 ELISA Development Kit（ELISAプレート構築キット）

ELISA Development Kitは、ELISAプレートをお客様ご自身で構築するキットで、ELISAプレートの構築に必要な補足用抗体、検出用抗体、スタンダードなどがセットされています。ELISAプレートの構築には少しの手間がかかりますが、Standardサイズでプレート約10枚分（約1,000ウェル分）を構築でき、固相化済みプレートの購入と比較すると大幅なコストダウンが期待できます。また、使用する分を作製できるため無駄が少なくなります。

製品容量は、約10枚分（約1,000ウェル分）のELISAプレートを構築できるStandardサイズの他にも、プレート約2枚分（約200ウェル分）のMiniサイズを用意している製品もあります。

• Human ELISA Development Kit
• Murine ELISA Development Kit
• Rat ELISA Development Kit

詳細情報　＞＞　Peprotech社WEBサイト

この製品に関するご意見・ご照会・お問合せはこちら

メーカー情報 

Enzyme-linked immunosorbent assay (ELISA) is a laboratory technique for quantifying the concentration of specific antigens or antibodies in cell lysates, sera, or other complex solutions. PeproTech’s ELISA Development Kits contain the key components required for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. Each kit contains the capture antibody, a biotinylated detection antibody, a calibrated antigen standard, the Avidin Peroxidase conjugate, and a kit-specific protocol.

■ ELISAプレート構築プロトコール

■ キット構成　例）Human IL-6 ELISA Development Kit 

• Capture Antibody:
  100 μg of antigen-affinity purified goat anti-hIL-6 + 2.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 ml sterile water for a concentration of 100 μg/ml.
• Detection Antibody:
  25 μg of biotinylated antigen-affinity purified goat anti-hIL-6 + 2.5 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 0.25 ml sterile water for a concentration of 100 μg/ml.
• Human IL-6 Standard:
  1 μg of recombinant hIL-6 + 2.2 mg BSA + 11.0 mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 ml sterile water for a concentration of 1 μg/ml.
• Avidin-HRP Conjugate:
  60 μl vial. Upon receipt, avidin-HRP conjugate should be aliquoted into ten 6 μl vials and stored at <-200C. Aliquots stored frozen at <-200C are stable for up to 2 years form date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.

■ 製品ラインアップ 

• Human ELISA Development Kit
• Murine ELISA Development Kit
• Rat ELISA Development Kit
• ELISA Buffer Kit

 ● Human ELISA Development Kit   

 ● Murine ELISA Development Kit   

 ● Rat ELISA Development Kit   

 ● ELISA Buffer Kit   

Catalog Number: 900-K00

• PBS:
  2 x 10ml per bottle 20x concentrated PBS solution
• Wash Buffer:
  2 x 250ml per bottle 20x concentrated Wash Buffer (1.0% TWEEN-20 in PBS)
• Blocking Buffer:
  2 x 200ml per bottle ready to use Blocking Buffer (1% BSA in PBS)
• Diluent:
  2 x 15ml per bottle 20x concentrated Diluent (1.0% TWEEN-20, 2.0% BSA in PBS)
• ABTS Liquid Substrate:
  2 x 60ml per bottle ready to use ABTS
• ELISA Microplates:
  10 x 96 well plates (Corning Product #3590)
• Sealing Film:
  50 Polyester Adhesive Sheets 

詳細情報　＞＞　Peprotech社WEBサイト 

この製品に関するご意見・ご照会・お問合せはこちら