長期間高い生存率を維持
GS21 Neural Supplement is Superior to B27® for Culturing Neurons
GS21 is the next generation serum-free neural media supplement, based on the formulation of NS21 Supplement, designed to improve the overall growth and performance of primary neurons1. GS21 is specifically optimized for the maturation and long-term viability of primary rat and mouse neurons in culture, without the need for co-culture with astrocyte feeder cells.
- Significantly improves long-term viability of rat and mouse primary neurons in vitro.
- Enhances neurite outgrowth of primary neurons in culture.
- Supports growth and maintenance of primary neurons at low or high cell density plating.
Significantly Higher Neuron Viability
Fig.1 - Higher neuron viability after 2 & 4 weeks in culture - Primary Rat Cortical Neurons (GSC-8220) cultured in GS21 supplemented culture media showed significantly higher neuron viability after 2 weeks, p=0.032 and 4 weeks, p=0.005 in culture, as compared to cells cultured in B27®. Neurons plated in 96-well plates were labeled with MAP2 (green), and Dapi (blue) to denote nuclei. Cells were imaged on a BD Pathway™ Bioimager after 1, 2 and 4 weeks in culture. Images were acquired using a 10x objective (0.4 NA). Data from n=4 wells is shown as mean ± SEM.
Fig.2 - GS21 Supplement supports 6-week cultured neurons - Primary Rat Cortical Neurons cultured in GS21 supplemented culture media remain viable after 6 weeks in culture. The above image shows healthy, mature primary neurons identified by MAP2 staining (green). Cells were imaged on a BD Pathway™ Bioimager after 6 weeks in culture. Images were acquired at 10x and 20x (inset) magnifications.
Improved Neurite Outgrowth
Fig.3 - Greater neurite outgrowth after 1,2 & 4 weeks in culture - Primary Neurons were cultured in GS21 supplemented neural base media. MAP2 labeling (top, left) was used to determine boundaries of the individual neuron cell bodies and associated neurites (colors are randomly assigned by the software). GS21 supplemented cultures showed significantly greater neurite lengths (top) and number of neurite segments (bottom) in culture, as compared to cultures supplemented with B27: neurite lengths, 1 week, (p=0.022), 2 week (p=0.017) and 4 week (p=0.023); and number of neurite segments (bottom) in 1 week, (p=0.036), 2 week (p=0.041) and 4 week (p=0.010). Images were acquired using a 10x objective (0.4 NA). Data from n=4 wells is shown as mean ± SEM.
1Chen Y, Stevens B, Chang J, Milbrandt J, Barres BA, Hell JW. NS21:re-defined and Modified Supplement B27 for Neuronal Cultures. J Neurosci Methods, 171 (2) 239-0247, 2008.
Technical Data
| Product Name | Cat. No. | Amount |
| GS21 Neural Supplement (50X) | GMS-3100 | 10 ml |