Cytoskeleton社 F-actin蛍光染色試薬 Acti-stain™ Fluorescent Phalloidins

Acti-stain™シリーズは、F-actin（アクチン）の検出に用いられるPhalloidin（ファロイジン）に、高輝度かつ退色耐久性に優れた色素をコンジュゲートした製品です。 

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メーカー情報 

The Acti-stain™ line of fluorescent phalloidins has been developed with an emphasis on creating exceptionally bright and stable probes at an economical price. Side-by-side comparisons with leading competing products demonstrate that you will enjoy savings while not sacrificing the quality of the stain when switching to an Acti-stain™ probe. Additionally, these probes are compatible with popular filter sets such as FITC, TRITC and Cy5. The combination of in-house manufacturing, stringent quality control and convenient packaging provides a great value. Give them a try and see for yourself.

Product Uses Include

• Stain F-actin in fixed cells
• Stabilize actin filaments in vitro
• Visualize actin filaments in vitro

Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures.

■ Biochemical characteristics of fluorescent phalloidins

 ● Acti-stain™ 488   

Swiss 3T3 cell stained with anti-vinculin (red), Dapi (blue nucleus) and F-actin is stained with Acti-stain™ 488 (green F-actin, Cat.# PHDG1).	Absorbance and fluorescence scan of Acti-stain™ 488. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 350-750 and 500-750 nm, respectively. Absorbance peaks at 500 nm and fluorescence at 550 nm.

 ● Acti-stain™ 555   

Swiss 3T3 cell with activated Rho, nucleus is stained with Dapi (blue) and F-actin is stained with Acti-stain™ 555 (red F-actin, Cat.# PHDH1).	Absorbance and fluorescence scan of Acti-stain™ 555. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 560-750 nm, respectively. Absorbance peaks at 560 nm and fluorescence at 575 nm.

 ● Acti-stain™ 670                                       

Mitotic Swiss 3T3 cell, F-actin stained with Acti-stain™ 670 (far-red, Cat. # PHDN1), nuclear DNA stained with Dapi (blue).	Absorbance and fluorescence scan of Acti-stain™ 670. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 300-750 and 600-750 nm, respectively. Absorbance peaks at 625 nm and fluorescence at 675 nm.

詳細情報　＞＞　Cytoskeleton社WEBサイト

この製品に関するご意見・ご照会・お問合せはこちら