2本鎖DNAの定量を超感度かつ広範囲で行う。
AccuBlue™ / AccuClear™
AccuAccuClear™ and AccuBlue™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts. Biotium offers three dsDNA quantitiation kits for different instruments and sample concentration ranges. The kits also differ in their tolerance of various contaminants; download the product protocols at www.biotium.com for details. The AccuClear™ Ultra High Sensitivity assay is based on a novel, next-generation DNA binding dye which offers unrivaled sensitivity and dynamic range compared to other DNA dyes.
AccuBlue™ Broad Range
• Linear range: 2-2000 ng dsDNA
• Blue fluorescence (Ex/Em: 350/460 nm)
• Assay can be extended to 4000 ng dsDNA with minor loss of linearity
• Blue fluorescence detection, compatible with fluorescence microplate reader
AccuBlue™ High Sensitivity
• Linear range: 0.2-100 ng dsDNA
• Green fluorescence (Ex/Em: 485/530 nm)
• Membrane-impermeable dye is non-toxic and non-mutagenic, for safer handling and easy disposal
• Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer such as Qubit®, or NanoDrop® fluorescence reader
AccuClear Ultra High Sensitivity™
• Linear range: 0.03-250 ng dsDNA
• Green fluorescence (Ex/Em: 460/507 nm)
• Unrivaled sensitivity and dynamic range
• Novel green fluorescent dye is a perfect match for blue LED excitation sources
• Green fluorescence detection compatible with fluorescence microplate reader, handheld fluorometer such as Qubit®, or NanoDrop® fluorospectrometer
Figure 1: Triplicate samples of calf thymus dsDNA, mouse liver RNA or salmon sperm ssDNA were assayed with the AccuBlue High Sensitivity dsDNA Quantitation Kit. The AccuBlue High Sensitivity Assay is selective for double-stranded DNA over RNA or single-stranded DNA.
Figure 2 Quantitate a large number of dsDNA samples in four easy steps with AccuBlue™ or AccuClear™.
AccuBlue™
High Sensitivity Dye A社 
Figure 7: The AccuBlue High Sensitivity reagent and Company A were diluted to working concentrations in HeLa cell cultures and incubated for 30 minutes. Company A readily bind nuclear DNA while no nuclear staining is evident with the AccuBlue HS reagent, illustrating its overall safety and lower toxicity due to its membrane impermeability.
| Ordering Information | |||
| Linear Range | Cat No. | Product Name | Kit Size |
| Broad Range 2-2000 ng |
31009-T | AccuBlue Broad Range dsDNA Quantitation Solution, Trial Size | 200 Assays |
| 31009 | AccuBlue Broad Range dsDNA Quantitation Solution | 1000 Assays | |
| 31007 | AccuBlue Broad Range dsDNA Quantitation Kit with 9 DNA standards | 1000 Assays | |
| High Sensitivity 0.2-100 ng |
31008-T | AccuBlue High Sensitivity dsDNA Quantitation Solution, Trial Size | 200 Assays |
| 31008 | AccuBlue High Sensitivity dsDNA Quantitation Solution | 1000 Assays | |
| 31006 | AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards | 1000 Assays | |
| Ultra High Sensitivity 0.03-250 ng |
31027-T | AccuClear Ultra High Sensitivity dsDNA Quantitation Solution, Trial Size | 200 Assays |
| 31027 | AccuClear Ultra High Sensitivity dsDNA Quantitation Solution | 1000 Assays | |
| 31028 | AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 7 DNA Standards | 1000 Assays | |
| 31029 | AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 1 DNA Standard | 4000 Assays | |